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The application of optical projection tomography to in-vivo
experiments is limited by specimen movement during the acquisition. We
present a set of mathematical correction methods applied to the acquired
data stacks to correct for movement in both directions of the image plane.
These methods have been applied to correct experimental data taken from
in-vivo optical projection tomography experiments in Caenorhabditis
elegans. Successful reconstructions for both fluorescence and white light
(absorption) measurements are shown. Since no difference between
movement of the animal and movement of the rotation axis is made, this
approach at the same time removes artifacts due to mechanical drifts and
errors in the assumed center of rotation.